Purification of Large Quantities of Coxiella burnetii Rickettsia by Density Gradient Zonal Centrifugation
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چکیده
منابع مشابه
Purification of large quantities of influenza virus by density gradient centrifugation.
New zonal centrifuges can conveniently process as much as five orders of magnitude (10(5)) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration...
متن کاملPurification of bacterial endotoxins by zonal centrifugation.
In this paper, we describe a cultivation procedure in large-capacity fermentors (200 liters) and the purification of the Salmonella crude extracts by means of zonal centrifugation in a sucrose gradient. Briefly, the endotoxin has been extracted from salmonellae with hypertonic solutions. The crude endotoxin was shown to contain several antigens, mainly r(1), r(2), r(3), or heterologous antigen....
متن کاملGenome size of the rickettsia Coxiella burnetii.
The genome size of Coxiella burnetii Nine Mile strain was determined by the method of initial rate of deoxyribonucleic acid renaturation. The mean value obtained was 1.04 X 10(9) daltons.
متن کاملImmunological properties of Rickettsia rickettsii purified by zonal centrifugation.
The properties of Rickettsia rickettsii purified from infected chicken yolk sacs or mouse L cell cultures by sucrose density gradient centrifugation in a zonal rotor were examined in various ways. Rickettsiae derived from both L cells and yolk sacs had similar compositions: about 12% nitrogen, 1.5% phosphorus, 5% carbohydrate, and 30% fatty acids. On a dry-weight basis, purified rickettsiae wer...
متن کاملLarge-scale purification of ribonucleic acid tumor viruses by use of continuous-flow density gradient centrifugation.
This report describes a 200- to 300-fold increase in the quantity of ribonucleic acud tumor virus particles previously isolated at one time by zonal centrifugation.
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1972
ISSN: 0003-6919
DOI: 10.1128/aem.23.5.1015-1022.1972